Figure 2

PGE ₂ and LTB ₄ production in response to L. longipalpis SGS during L. infantum infection. C57BL/6 mice were injected i.p. with saline (control), L. infantum and/or SGS. One, 3 and 6 hours after stimulation, peritoneal cavities were washed and cells were harvested. The cells were then incubated with A23187 (0.5 mM) for 15 min at 37°C to evaluate LTB₄ and PGE₂ production. The concentrations of PGE₂(A) and LTB₄(B) in the supernatant were measured by ELISA. (C) Shows the PGE₂/LTB₄ ratio. Area Under Curve (AUC) of the kinetic of PGE₂(D), LTB₄(E) and PGE₂/LTB₄ ratio (F). The data are the means and SEM from an experiment representative of three independent experiments. n = 5 per group. (*, p < 0.05 and Mann–Whitney test, two-tailed).