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Figure 2 | Parasites & Vectors

Figure 2

From: Prevalence of Rickettsiales in ticks removed from the skin of outdoor workers in North Carolina

Figure 2

PCR-RLB hybridization results for 23S-5S IGS fragments amplified from genomic DNA extracted from Rickettsia controls and A . americanum ticks. Next to the identification code of tick samples, Rickettsia species identifications as determined by the RLB hybridization patterns are shown in parentheses. Ra = R. amblyommii and Rm = R. montanensis. An example of hybridization patterns for some biotin-labeled A. americanum 23S-5S DNAs are presented in the lower half the figure. All of the ticks exhibited strong hybridization to the Rickettsia genus level (GP-RICK) and SFG (GP-SFG) probes, indicating that they were infected with a spotted fever group Rickettsia species. All but one 23S-5S DNA samples hybridized with probe P-AMB, indicating that these ticks were infected with R. amblyommii. Tick DNA sample 2FT365 hybridized with probe P-MAS/MON, but failed to hybridize to P-MAS/RHIPI, indicating that the tick was infected with R. montanensis.

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