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Figure 2 | Parasites & Vectors

Figure 2

From: Sm16, a major component of Schistosoma mansoni cercarial excretory/secretory products, prevents macrophage classical activation and delays antigen processing

Figure 2

Sm16 is enriched in pellet fraction of cercarial E/S products. Three preparations of 0-3hRP were fractionated by ultracentrifugation and the pellet fractions restored to the original volume of each preparation. (A) Protein content of each fraction expressed as a percentage of the total protein present in the original preparation. Bars = mean + SEM; statistical significance was tested using two tailed t-test (*** = p < 0.001). (B) SDS PAGE gel of 0-3hRP fractions (5, 10 and 20 μg) stained for protein. Black arrows highlight bands identified by mass spectrometry. (C) Equivalent volumes of 0-3hRPS (78 μg) and 0-3hRPP (10 μg) based on the original preparation, were processed for Western blot analysis alongside rSm16 (1 μg) probed using rabbit anti-rSm16 antibody, estimated as relative concentration of Sm16 / μg 0-3hRP. Bars are mean + SEM, two tailedt-test show statistically significant differences (**** = p < 0.0001).

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