Figure 5From: Sm16, a major component of Schistosoma mansoni cercarial excretory/secretory products, prevents macrophage classical activation and delays antigen processing Uptake of fluorescently labelled rSm16 by BMMΦs. Representative confocal images of (A) BMMΦs exposed to labelled rSm16AF594 (red) stained with DAPI (blue) within EEA-1+ endosomes (green) 10 min and 100 min after ligand stimulation. (B) Insert showing EEA-1+ endosomes containing rSm16AF594 (2 μm x 2 μm) (C) BMMΦs exposed for 100 min to rSm16AF594 (red) and DEXTRANFITC (green) washed and imaged after 10, 60 and 100 min. (63x objective, scale bar = 10 μm; acquired using a Zeiss LSM 710 invert microscope).Back to article page