Figure 2From: Alteration of immunoproteome profile of Echinococcus granulosus hydatid fluid with progression of cystic echinococcosis Global immunoproteome characteristics of CE2 HF. (A) Immunoproteome analysis of HF against pooled sera of different CE stages. The patients were classified by typical US findings [4]. CE2 HF was separated by 2-DE, transferred to nitrocellulose membranes, and probed with pooled CE1-CE5 sera (n = 3 per group) as described in the Methods section. Immunoreactions of EgAg5 (purple), EgAgB1 subunit (blue), EgAgB1 (red), EgAgB subunit 2 (khaki), EgAgB2 (brown), and EgAgB4 (green) are indicated. All signals were detected by ECL after 2 min exposure. Spots at ca. 24 kDa could not be identified on 2-DE gel, but showed immunoreactivity (box, left panel). Each corresponding band from CE1 and CE2 HF was independently analyzed by nano-LC-ESI-MS/MS. (B) Identification of proteins within box in A by PMF. (C) Immunoreactivity of each spot in Figure 1A (left panel) was compared to the volumes of the corresponding protein spots. The open bars indicate the relative volumes (left Y-axis) and normalized protein volumes (right Y-axis) in the CBB stained gels. The solid bars indicate the relative volumes of the immunoblot.Back to article page