Global immunoproteome characteristics of CE2 HF. (A) Immunoproteome analysis of HF against pooled sera of different CE stages. The patients were classified by typical US findings . CE2 HF was separated by 2-DE, transferred to nitrocellulose membranes, and probed with pooled CE1-CE5 sera (n = 3 per group) as described in the Methods section. Immunoreactions of EgAg5 (purple), EgAgB1 subunit (blue), EgAgB1 (red), EgAgB subunit 2 (khaki), EgAgB2 (brown), and EgAgB4 (green) are indicated. All signals were detected by ECL after 2 min exposure. Spots at ca. 24 kDa could not be identified on 2-DE gel, but showed immunoreactivity (box, left panel). Each corresponding band from CE1 and CE2 HF was independently analyzed by nano-LC-ESI-MS/MS. (B) Identification of proteins within box in A by PMF. (C) Immunoreactivity of each spot in Figure 1A (left panel) was compared to the volumes of the corresponding protein spots. The open bars indicate the relative volumes (left Y-axis) and normalized protein volumes (right Y-axis) in the CBB stained gels. The solid bars indicate the relative volumes of the immunoblot.