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Table 2 Primers used for vector-borne pathogens detection by PCR in the study

From: Diagnosis and incidence risk of clinical canine monocytic ehrlichiosis under field conditions in Southern Europe

Gene target PCR target Name Primer sequence Fragment length Reference
Mitosin gene Canis familiaris CAN-F 5′-CTTGTCACGGTAAGGTTC-3′ 290-bp [14]
CAN-R 5′-CTGATGTATTTCCTGCACCAAG-3′
Vir-B9 protein gene Ehrlichia canis Ehr1401F 5′-CCATAAGCATAGCTGATAACCCTGTTACAA-3′ 380-bp [15]
Ehr1780R 5′-TGGATAATAAAACCGTACTATGTATGCTAG-3′
GroEL gene Anaplasma platys GroAplatys-35 s 5′-AGCGTAGTCCGATTCTCCAGTTTT-3′ 515-bp [16]
GroAplatys-550as 5′-TCGCCGTTAGCAGAGATGGTAG-3′
AnkA gene Anaplasma phagocytophilum AnkAP-2074s 5′-GGCAAATGAGGCAAGTAACC-3′ 741-bp [16]
AnkAP-2815as 5′-GCCACTACCCAAGGATGATAG-3′
18S rDNA Babesia spp Ba103F 5′-CCAATCCTGACACAGGGAGGTAGTGACA-3′ 612-bp [15]
Ba721R 5′-CCCCAGAACCCAAAGACTTTGATTTCTCTCAAG-3′
18S rDNA Hepatozoon canis Hep F 5′-ATACATGAGCAAAATCTCAAC-3′ 666-bp [17]
Hep R 5′-CTTATTATTCCATGCTGCAG-3′
18S rDNA Leishmania spp R221 5′-GGTTCCTTTCCTGATTTACG-3′ 603-bp [18]
R332 5′-GGCCGGTAAAGGCCGAATAG-3′