First report of Theileria and Anaplasma in the Mongolian gazelle, Procapra gutturosa

Li, Youquan; Chen, Ze; Liu, Zhijie; Liu, Junlong; Yang, Jifei; Li, Qian; Li, Yaqiong; Ren, Qiaoyun; Niu, Qingli; Guan, Guiquan; Luo, Jianxun; Yin, Hong

doi:10.1186/s13071-014-0614-3

Parasites & Vectors

Table 1 Sequences of the oligonucleotide primers used in this study

From: First report of Theileria and Anaplasma in the Mongolian gazelle, Procapra gutturosa

Pathogen	Target gene	Primers		Final amplicon size (bp)	References
Pathogen	Target gene	Primer name	Oligonucleotide sequences (5’-3’)	Final amplicon size (bp)	References
Anaplasma & Ehrlichia	16S rRNA	EC9	TACCTTGTTACGACTT	1462	Kawahara et al., 2006 [10]
Anaplasma & Ehrlichia	16S rRNA	EC12A	TGATCCTGGCTCAGAACGAACG	1462	Kawahara et al., 2006 [10]
A. bovis	16S rRNA	AB1f	CTCGTAGCTTGCTATGAGAAC	551	Kawahara et al., 2006 [10]
A. bovis	16S rRNA	AB1r	TCTCCCGGACTCCAGTCTG	551	Kawahara et al., 2006 [10]
A. phagocytophilum	16S rRNA	SSAP2f	GCTGAATGTGGGGATAATTTAT	641	Kawahara et al., 2006 [10]
A. phagocytophilum	16S rRNA	SSAP2r	ATGGCTGCTTCCTTTCGGTTA	641	Kawahara et al., 2006 [10]
A. marginale	msp4	Amargmsp4 F	CTGAAGGGGGAGTAATGGG	344	Torina et al., 2012 [11]
A. marginale	msp4	Amargmsp4 R	GGTAATAGCTGCCAGAGATTCC	344	Torina et al., 2012 [11]
A. ovis	msp4	Aovismsp4 F	TGAAGGGAGCGGGGTCATGGG	347	Torina et al., 2012 [11]
A. ovis	msp4	Aovismsp4 R	GAGTAATTGCAGCCAGGGACTCT	347	Torina et al., 2012 [11]
Hemoparasite	18S rRNA	Primer A	AACCTGGTTGATCCTGCCAGT	1750	Medlin et al., 1988 [14]
Hemoparasite	18S rRNA	Primer B	GATCCTTCTGCAGGTTCACCTAC	1750	Medlin et al., 1988 [14]
Theileria	18S rRNA	989	AGTTTCTGACCTATCAG	1100	Allosop et al., 1993 [15]
Theileria	18S rRNA	990	TTGCCTTAAACTTCCTTG	1100
T. luwenshuni	18S rRNA	Tl310	GGTAGGGTATTGGCCTACTGA	340	Yin et al., 2008 [16]
T. luwenshuni	18S rRNA	Tl680	TCATCCGGATAATACAAGT	340	Yin et al., 2008 [16]
Babesia	18S rRNA	Babesia F	TGTCTTGAATACTT(C/G)AGCATGGAA	950	Ramos et al., 2010 [17]
Babesia	18S rRNA	Babesia R	CGACTTCTCCTTTAAGTGATAAC	950	Ramos et al., 2010 [17]

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ISSN: 1756-3305

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