REL2-F silencing suspends the hemozoin effect. Female mosquitoes were silenced using dsRNA specific for REL2-F or control-dsRNA against β2M.
(A) Silencing efficiency was measured both at the fat body (Fb) and the midgut (Mg). Differences and P values: *(p < 0.05), using the Student t test. (B) Silenced mosquitoes were treated with sHz or PBS-control and infected with P. berghei; oocysts were counted 8 days post-infection. Horizontal red bars represent the median. N, number of female mosquitoes; n, number of independent experiments; IR, infection rate (%). (C) Relative induction fold change of REL2-F and six other immune related genes measured in the fat body following REL2-F silencing and subsequent sHz treatment, determined by qRT-PCR comparison to PBS-treated controls.