Identification of recombinant Tspst protein. A. SDS-PAGE analysis of recombinant Tspst protein (rTspst). M: protein molecular weight marker; 1: T. spiralis ML crude antigens (15 μg/lane); 2: T. spiralis ML ES antigens (15 μg/lane); 3: rTspst (3 μg/lane) purified by Amylose Pre-packed Column. B. Western blot analysis of rTspst antigenicity. The T. spiralis ML crude antigens (1), ES antigens (2), and rTspst (3) were recognized by sera of mice infected with T. spiralis at 30 dpi. The native Tspst protein in crude antigens (4) and rTspst (6) were recognized by anti-rTspst serum, but the ES antigens (5) were not recognized by anti-rTspst serum. The T. spiralis crude antigens (7), ES antigens (8), and rTspst (9) were not recognized by normal mouse sera.