Figure 4
Phylogenetic relationships of B. schroederi Bsc -GSTĪ and other classes of GSTs. An unrooted phylogenetic tree was inferred by neighbor-joining (NJ) analysis. Representative GSTs from eight classes: Îą, Ī, Ί, θ, Ξ, Îŧ, Ī, and Micro. The tree was constructed from a multiple sequence alignment performed using ClustalW2 and plotted using MEGA 5.0. Based on the GTRâ+âIâ+âG model, 1,000 bootstrap replicates were run to calculate the percentage reliability for each node, and only values âĨ50% are shown. The scale indicates an estimate of substitutions per site, using the optimized model setting. The protein sequences used in the tree are listed as follows, with their GenBank or SwissProt accession numbers: Bos taurus alpha GST (Bta-GSTAlpha), Q28035; Ovis aries GST (Oar-GST), Q9XS30; Sus scrofa alpha GST (Ssc-GSTAlpha), P51781; Homo sapiens alpha GST (Hsa-GSTAlpha), P08263; Mus musculus alpha GST (Mmu-GSTAlpha), Q6P8Q0; Ailuropoda melanoleuca alpha GST (Ame-GSTAlpha), XP_002923004; Drosophila melanogaster sigma GST (Dme-GSTSigma), P41043; Onchocerca volvulus sigma GST (Ovo-GSTSigma), P46434; H. sapiens sigma GST (Hsa-GSTSigma), O60760; Canis familiaris prostaglandin D synthase (Caf-Prostaglandin), NP_001186973; M. musculus sigma GST (Mmu-GSTSigma), Q9JHF7; Rattus norvegicus GST (Rno-GST),O35543; Gallus gallus sigma GST (Gga-GSTSigma), O73888; Xenopus laevis GST (Xla-GST), AAH53774.1; C. elegans sigma GST N (Cel-GSTNSigma), AAB65417; B. schroederi sigma GST (Bsc-GSTSigma), KM435257; A. suum sigma GST (Asu-GSTSigma), P46436; C. elegans sigma GST (Cel-GSTSigma), P91253; A. caninum sigma GST (Aca-GSTSigma), AAT37718; N. americanus sigma GST1 (Nam-GST1Sigma), ACX53261; H. contortus GST (Hco-GST), Q9NAW7; N. americanus sigma GST2 (Nam-GST2Sigma), ACX53262; N. americanus sigma GST3 (Nam-GST3Sigma), ACX53263; Clonorchis sinensis 28-kDa GST (Csi-28GST), O97096; Opisthorchis viverrini sigma GST (Ovi-GSTSigma), AAL23713; Fasciola hepatica sigma GST (Fhe-GSTSigma), ABI79450; Paragonimus westermani 28-kDa sigma GST (Pwe-28GSTSigma), AAB63382; Schistosoma haematobium 28-kDa sigma GST (Sha-28GSTSigma), AAA29892; Schistosoma bovis 28-kDa sigma GST (Sbo-28GSTSigma), AAA29893; C. elegans omega GST (Cel-GSTOmega), P34345; H. sapiens omega GST (Hsa-GSTOmega), P78417; M. musculus omega GST (Mmu-GSTOmega), O09131; F. hepatica omega GST (Fhe-GSTOmega), AFX98104; Schistosoma mansoni omega GST (Sma-GSTOmega), Q86LC0; D. melanogaster theta GST (Dme-GSTTheta), P20432; M. musculus theta GST (Mmu-GSTTheta), Q91X50; H. sapiens theta GST (Hsa-GSTTheta), P30711; A. melanoleuca theta GST (Ame-GSTTheta), XP_002925113; H. sapiens zeta GST (Hsa-GSTZeta),O43708; M. musculus zeta GST (Mmu-GSTZeta), Q9WVL0; D. melanogaster zeta GST (Dme-GSTZeta), Q9VHD3.1; Bombyx mori zeta GST4 (Bmo-GST4Zeta), ABC79691;C. elegans GST 43 (Cel-GST43), Q9N4H6; H. sapiens mu GST (Hsa-GSTMu), P09488; B. taurus mu GST (Bta-GSTMu), Q9N0V4; S. scrofa mu GST (Ssc-GSTMu), Q000H8; A. melanoleuca mu GST (Ame-GSTMu), XP_002919259; M. musculus mu GST (Mmu-GSTMu), P10649; Schistosoma japonicum 26-kDa mu GST (Sja-26GSTMu), P08515; S. mansoni 26-kDa mu GST (Sma-26GSTMu), P15964; C. sinensis mu GST (Csi-GSTMu), AAB46369; F. gigantica mu GST (Fgi-GSTMu), AAD23997; F. hepatica mu GST (Fhe-GSTMu), AHC02709; Capra hircus pi GST (Chi-GSTPi), Q9TTY8; B. taurus pi GST (Bta-GSTPi), P28801; M. musculus pi GST (Mmu-GSTPi), P19157; H. sapiens pi GST (Hsa-GSTPi), P09211; S. scrofa pi GST (Ssc-GSTPi), P80031; C. elegans pi GST (Cel-GSTPi), P10299; Dirofilaria immitis pi GST (Dim-GSTPi), P46426; Brugia malayi pi GST (Bma-GSTPi), CAA73325; O. volvulus pi GST (Ovo-GSTPi), P46427; D. melanogaster micro GST (Dme-GST-Micro), AAC98692; M. musculus micro GST (Mmu-GST-Micro), NP_064330.