Figure 5From: Cloning and characterization of a novel sigma-like glutathione S-transferase from the giant panda parasitic nematode, Baylisascaris schroederi SDS-PAGE and western blotting analysis of recombinant Bsc -GSTĪ (r Bsc -GSTĪ) protein. M, molecular weight marker in kDa; lane 1, IPTG-induced E. coli BL21 (DE3) lysate; lanes 2â3, different concentrations of purified rBsc-GSTĪ after dialysis; lane 4, the refolded protein after treatment with the vacuum freeze-drying technique; lanes 5â8: refolded rBsc-GSTĪ probed with rabbit immune serum against B. schroederi (experimental group, lane 5), naïve rabbit serum (negative control, lane 6), anti-rBsc-GSTĪ mouse serum (positive control, lane 7), and pre-immune mouse serum (negative control, lane 8), respectively. The protein was stained with Coomassie Blue R250 in the gel (lanes 1â4), while the protein bound to serum samples was detected using NBT/BCIP in the immunoblotting (lanes 5â8). Two white arrows indicate the location of rBsc-GSTĪ-specific bands.Back to article page