Figure 5

SDS-PAGE and western blotting analysis of recombinant Bsc -GSTσ (r Bsc -GSTσ) protein. M, molecular weight marker in kDa; lane 1, IPTG-induced E. coli BL21 (DE3) lysate; lanes 2–3, different concentrations of purified rBsc-GSTσ after dialysis; lane 4, the refolded protein after treatment with the vacuum freeze-drying technique; lanes 5–8: refolded rBsc-GSTσ probed with rabbit immune serum against B. schroederi (experimental group, lane 5), naïve rabbit serum (negative control, lane 6), anti-rBsc-GSTσ mouse serum (positive control, lane 7), and pre-immune mouse serum (negative control, lane 8), respectively. The protein was stained with Coomassie Blue R250 in the gel (lanes 1–4), while the protein bound to serum samples was detected using NBT/BCIP in the immunoblotting (lanes 5–8). Two white arrows indicate the location of rBsc-GSTσ-specific bands.