Figure 6

Localization of endogenous Bsc -GSTσ in female adult B. schroederi using immunofluorescence. Worms were fixed in paraformaldehyde and embedded in paraffin as described in Methods. The sections (5-μm thickness) were incubated with either mouse anti-rBsc-GSTσ serum (1:100; panels A-F) or pre-immune serum at (1:100; panels G-L). The endogenous Bsc-GSTσ becomes visible with fluorescein isothiocyanate (FITC)-labeled goat anti-mouse IgG (1:100). White arrows indicate antibody-labeled regions. Different magnifications are used to highlight these regions and they include 10× (A), 20× (B, C, G, and I), and 40× (D-F, H, and J-L). Abbreviations: Hd, hypodermis; Mu, muscle; Lc, lateral chord; Gu, gut; Gue, gut epithelium; Mv, microvilli; Ov, ovary; Ut, uterus; Od, oviduct; Ode, oviduct epithelium; Nee, non-embryonated eggs; Ew, egg wall.