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Figure 4 | Parasites & Vectors

Figure 4

From: Immunosuppressive effects of Amblyomma cajennense tick saliva on murine bone marrow-derived dendritic cells

Figure 4

The effect of tick saliva on TNF-α, IL-12 p40, IL-6 and IL-10 production in DCs stimulated with LPS. Bone marrow cells derived from C57BL/6 mice were collected and cultured for 7 days in the presence of GM-CSF to allow differentiation into DCs. DCs were then pre-incubated with saliva (diluted 1:10, 1:30, 1:100 1:300 and 1:1000 v/v) from A. cajennense for 1 hour. DCs were then stimulated for an additional 18 hours with LPS (100 ng/ml). The culture supernatant was then collected and analyzed to detect TNF-α (A), IL-12 p40(B), IL-6 (C) and IL-10 (D) by ELISA. Bars represent the mean ± SD production level of TNF-α, IL-12 p40, IL-6 and IL-10 in cultured DCs from triplicate experiments. *: p < 0.05 compared with DCs cultured with LPS.

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