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Figure 6 | Parasites & Vectors

Figure 6

From: Immunosuppressive effects of Amblyomma cajennense tick saliva on murine bone marrow-derived dendritic cells

Figure 6

The effect of tick saliva on viability of DCs. Bone marrow cells derived from C57BL/6 mice were collected and cultured for 7 days in the presence of GM-CSF to allow differentiation into DCs. DCs were then incubated with either saliva (diluted 1:10, 1:30, 1:100 1:300 and 1:1000 v/v) from A. cajennense for 18 hours. After that, the cells were collected and stained with Annexin V and propidium iodide to evaluate apoptosis by flow cytometry. Cell viability is shown in dot plots (A), where negative control cells are colored red and Annexin-V-FITC and Propidium Iodide stained cells are colored black (B). Bars represent the mean ± SD number of viable DCs (Annexin V-/PI-) from duplicate experiments.

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