SDS-PAGE and Western blot analysis of r
14-3-3 expressed in
BL21. (A) Bacterial lysates or purified recombinant protein were analyzed by SDS–PAGE and stained with Coomassie blue. Lane M, protein marker; lane 1, uninduced E. coli lysate; lane 2, IPTG-induced E. coli lysate; lane 3, insoluble fraction of induced E. coli lysate ; lane 4, soluble fraction of induced E. coli lysate ; lane 5, rTs14-3-3 purified by Ni-affinity chromatography. (B) Western blot analysis of rTs14-3-3. The rTs14-3-3 was transferred on individual lane and recognized by T. spiralis infected swine sera at 7 dpi (lane 1); T. spiralis infected mouse sera at 7 dpi (lane 2) and anti-His monoclonal antibody (lane 4). (C). The rTs14-3-3 was recognized by sera of swine infected with T. spiralis for 7 days (lane 1); 14 days (lane 2); 21 days (lane 3) and 28 days ( lane 4) or by anti-His antibody as control (lane 5). D. Western blot analysis of native Ts14-3-3 in adult extracts (lane 1) and in muscle larval extracts (lane 2) of T. spiralis by antibody against rTs-14-3-3.