Table 3 The PCR-based diagnostic test CF700 revealed the presence of C. faba or closely related species in benthos obtained at sites of focal occurrence of adult C. faba individuals

Mollusca:

Bythinella austriaca

1

59

0

0

-

Bythinella austriaca

2

10

1

10

1066 (KM594114)

Turbellaria:

Dendrocoelum lacteum

1

1

0

0

-

Dugesia gonocephala

1

4

0

0

-

Nematoda:

Gordius sp.

1

3

0

0

-

Crustacea:

Gammarus fossarum

1

121

19

16

250, 252, 257 (KC543332), 260 (KC543333), 266, 267, 272, 275, 283, 287, 288, 291, 198, 305, 306, 307, 316, 318, 319 (KC543334)

Gammarus fossarum

2

20

0

0

-

Gammarus fossarum

6

81

1

1

486 (KC543343)

Plecoptera:

Isoperla grammatica

1

18

2

11

776, 786 (KC543346)

Isoperla sp.

2

20

11

55

983-990, 991 (KC543349), 992 (KC543350), 1009

Leuctra braueri

1

3

0

0

-

Leuctra prima

1

1

0

0

-

Leuctra teriolensis × rauscheri

1

3

0

0

-

Perla bipunctata

6

1

0

0

-

Perla bipunctata

1

24

10

42

799, 800, 804, 806, 808, 818 (KC543347), 820, 822 (KC543348), 823, 826

Perla burmeisteriana

6

3

0

0

-

Perla sp.

2

20

6

30

1047, 1049, 1053, 1058, 1059, 1062

Protonemura auberti

6

7

0

0

-

Ephemeroptera:

Baetis alpinus

1

17

0

0

-

Ecdyonurus venosus

1

26

11

42

369, 376 (KC543339), 379 (KC543340), 381, 384, 387, 389, 391 (KC543341), 392, 393, 394

Ecdyonurus venosus

6

18

0

0

-

Ecdyonurus sp., juv.

6

4

0

0

-

Epeorus assimilis

6

1

0

0

-

Habroleptoides modesta

1

1

0

0

-

Rhithrogena carpatoalpina

6

1

0

0

-

Rhithrogena picteti × iridina

1

83

27

33

325, 326, 327, 330, 332 (KC543335), 333 (KC543336), 335 (KC543337), 336 (KC543338), 339, 340, 342, 349, 350, 353, 356, 359, 360, 362, 363, 364, 365, 367, 430 (KC543342), 651 (KC543344), 668, 694, 695 (KC543345)

Rhithrogena sp.

2

20

2

10

-

Seratella ignita

6

1

0

0

-

Trichoptera:

Drusus discolor

1

1

0

0

-

Drusus sp.

1

5

0

0

-

Hydropsyche instabilis

6

1

1

100

572

Philopotamus ludificatus

6

4

0

0

-

Plectrocnemia conspersa

6

7

0

0

-

Potamophylax luctuosus

6

3

0

0

-

Potamophylax sp., juv.

6

2

0

0

-

Rhyacophila hirticornis

1

1

0

0

-

Rhyacophila polonica

1

12

0

0

-

Rhyacophila polonica

6

1

0

0

-

Rhyacophila tristis

1

1

0

0

-

Rhyocophila hirticornis

1

3

0

0

-

Wormaldia copiosa

1

3

0

0

-

Diptera:

Dicranota sp.

1

1

0

0

-

Hexatoma sp.

1

1

0

0

-

Coleoptera:

Elmis aenea

6

1

0

0

-

Aves (controls from definitive hosts):

Hirundo rustica

827 (KM594115)

Erithacus rubecula

865 (KM594116)

Sylvia atricapilla

866 (KM594117)

Regulus regulus

870 (KM594118)