Intermediate hosts of the trematode Collyriclum faba (Plagiochiida: Collyriclidae) identified by an integrated morphological and genetic approach

Table 5 Morphological identification of trematode intermediate stages in ethanol-fixed specimens from cohorts examined in part by DNA sequencing in Table 3 , collected at sampling site 2 (as defined in Table 1 )

Species	N examined	N positive	Prevalence [%]	Specification of metacercariae	N of individuals	Number of metacercariae per host individual	Location in the host
Gammarus fossarum	167	0	0
Rhitrogena spp. (immature)	101	0	0
Isoperla spp. (immature)	35	2	6	Large	1	2	Abdomen
				Large	1	1	Thorax
Perla marginata (immature)	12	6	50	Very large	6	1.3 ± 0.5	Abdomen
				Medium	1^*	1	Abdomen
Perla marginata (imago)	15	7	47	Very large	6	3.0 ± 1.1	Abdomen
				Medium	1^**	1	Abdomen
				Small	6^†	4.8 ± 3.1	Abdomen

^*Mixed infection of the larva of Perla marginata with a single medium-sized metacercaria and two very large metacercariae. ^**Mixed infection of the imago of Perla marginata with a single medium-sized, four very large and nine small metacercariae. † Mixed infection of imagos of Perla marginata with small and very large metacercariae in five of the six cases.

ISSN: 1756-3305