Figure 1

MAPIA with recombinant Leishmania infantum antigens. Images of strips containing printed individual antigens. Strips were incubated with serum samples from L. infantum-positive dogs (lanes 1, 3–13), serum from a normal control (uninfected) dog (lane 2), and the positive standard control serum pool (lane 14). The optimum concentration of each antigen wereLci1 = 0.236 mg/mL, Lci2 = 0.222 mg/mL, Lci3 = 0.530 mg/mL, Lci4 = 0.055 mg/mL, Lci5 = 0.139 mg/mL, Lci6 = 0.347 mg/mL, Lci7 = 0.097 mg/mL, Lci8 = 0.125 mg/mL, Lci10 = 0.139 mg/mL, Lci11 = 0.055 mg/mL, Lci12 = 0.236 mg/mL, Lci13 = 0.180 mg/mL, L. major lysate = 0.7620 mg/mL, recombinant CRA&FRA T. cruzi proteins = 0.290 mg/mL and protein A solution = 0.200 mg/mL. Antibody reactivity was detected as described in Methods. The test bands were visually read by two independent operators. Any visible band in the test area (in addition to the control line) was considered a positive reaction.