Table 1 Sensitivity and specificity of MAPIA with recombinant antigens of Leishmania infantum for the serodiagnosis of canine visceral leishmaniasis
Study groups (n) | Reactivity with individual L. infantum antigens (number of positive sera) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lci1A | Lci2B | Lci3 | Lci4 | Lci5 | Lci6 | Lci7 | Lci8 | Lci10 | Lci11 | Lci12 | Lci13 | Any antigen | |
Dogs without signs of VL (16) | 3 | 2 | 4 | 6 | 4 | 1 | 2 | 1 | 4 | 2 | 6 | 1 | 9 |
Dogs with signs of VL (41) | 29 | 31 | 8 | 15 | 15 | 1 | 9 | 4 | 3 | 8 | 13 | 3 | 37 |
Total (57) | 32 | 33 | 12 | 21 | 19 | 2 | 11 | 5 | 7 | 10 | 19 | 4 | 46 |
% Sensitivity | 56 | 58 | 21 | 37 | 33 | 4 | 19 | 9 | 12 | 18 | 33 | 7 | 81 |
Normal control (40) | 0 | 1 | 2 | 2 | 2 | 1 | 3 | 1 | 2 | 2 | 4 | 3 | 6 |
Dermal leishmaniasis* (10) | 6 | 3 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 6 |
Trypanosomiasis* (10) | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 3 |
Babesiosis* (10) | 1 | 2 | 3 | 3 | 3 | 0 | 1 | 0 | 3 | 1 | 2 | 2 | 4 |
Ehrlichiosis* (11) | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 2 |
Total (81) | 8 | 7 | 7 | 11 | 9 | 1 | 7 | 1 | 7 | 3 | 10 | 6 | 21 |
% Specificity | 90 | 91 | 91 | 86 | 89 | 99 | 91 | 99 | 91 | 96 | 88 | 93 | 74 |