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Table 2 PLS cross-validation, % accuracy of species predictions using NIRS on preserved and fresh An. gambiae s.s. and An. Arabiensis samples

From: Evaluating preservation methods for identifying Anopheles gambiae s.s. and Anopheles arabiensis complex mosquitoes species using near infra-red spectroscopy

Storage duration (weeks) Fresh 4°C −20°C Desiccated in silica gel RNA later ® room temp. RNA later ® at 4°C RNA later ® at −20°C
An. arabiensis        
0 91.8 . . . . . .
1   96.3 94.8 94.8 82.8 87.4 87.8
4   83.3 91.1 88.9 78.5 85.1 83.1
10   90.8 69.6 80.7 68.7 70.6 72.9
32   83.7 83.7 80.7 72.2 80.0 71.1
50   ** 92.6 90.4 84.4 88.9 93.3
An. gambiae        
0 91.0 . . . . . .
1   97.3 98.5 85.2 90.2 94.0 94.6
4   91.9 91.8 88.4 78.8 83.7 82.6
10   94.1 74.2 81.5 69.6 69.1 69.5
32   87.8 79.3 89.7 80.0 75.9 70.4
50   ** 92.6 89.6 63.7 75.6 77.0
Average 91.4 90.7 86.8 87.0 76.9 81.0 80.2
  1. Caption: **Samples refrigerated at 4°C for 50 weeks were not suitable for scanning.