Investigation of tick-borne bacteria (Rickettsia spp., Anaplasma spp., Ehrlichia spp. and Borrelia spp.) in ticks collected from Andean tapirs, cattle and vegetation from a protected area in Ecuador

Pesquera, Cristina; Portillo, Aránzazu; Palomar, Ana M; Oteo, José A

doi:10.1186/s13071-015-0662-3

Table 1 PCR primer pairs used in this study

From: Investigation of tick-borne bacteria (Rickettsia spp., Anaplasma spp., Ehrlichia spp. and Borrelia spp.) in ticks collected from Andean tapirs, cattle and vegetation from a protected area in Ecuador

	Target gene	Primer name	Primer sequence (5’➜ 3’)	Fragment size (bp)	Tm (°C)	Reference
Tick species	16S rRNA	16S + 1	CTGCTCAATGATTTTTTAAATTGCTGTGG	456	48	[14]
Tick species	16S rRNA	16S – 1	CCGGTCTGAACTCAGATCAAGT		54	[14]
	12S rRNA	T1B	AAACTAGGATTAGATACCCT	338	51	[15]
	12S rRNA	T2A	AATGAGAGCGACGGGCGATGT		53	[15]
	ITS2	RIB-4 F	CCATCGATGTGAAYTGCAGGACA	800	55	[16]
	ITS2	RIB-R	GTGAATTCTATGCTTAAATTCAGGGGGT	800	55	[16]
Pan- bacterial	16S rRNA	fD1	AGAGTTTGATCCTGGCTCAG	1500	60	[17]
Pan- bacterial	16S rRNA	rP2	ACGGCTACCTTGTTACGACTT	1500	60	[17]
*Rickettsia* spp.	gltA	RpCS.877p	GGGGGCCTGCTCACGGCGG	1019	65	[18]
	gltA	RpCS1258n	ATTGCAAAAAGTACAGTGAACA	1019	65	[18]
	gltA (5’ end)	CS-78	GCAAGTATCGGTGAGGATGTAAT GCTTCCTTAAAATTCAATAAATCAGGAT	401	48	[19]
	gltA (5’ end)	CS-323	GCAAGTATCGGTGAGGATGTAAT GCTTCCTTAAAATTCAATAAATCAGGAT	401	48	[19]
	ompA (semi-nested)	Rr190.70p	ATGGCGAATATTTCTCCAAAA	631	46	[20]
		Rr190.701n	GTTCCGTTAATGGCAGCATCT	631	46	[20]
		Rr190.70p	ATGGCGAATATTTCTCCAAAA	532	48	[21]
		Rr190.602n	AGTGCAGCATTCGCTCCCCCT	532	48	[21]
	ompB (nested)	rompB OF	GTAACCGGAAGTAATCGTTTCGTAA	511	54	[22]
		rompB OR	GCTTTATAACCAGCTAAACCACC	511	54
		rompB SFG IF	GTTTAATACGTGCTGCTAACCAA	420	56
		rompB SFG/TG IR	GGTTTGGCCCATATACCATAAG	420	56
	sca4	D1f	ATGAGTAAAGACGGTAACCT	928	50	[23]
	sca4	D928r	AAGCTATTGCGTCATCTCCG	928	50	[23]
	16S rRNA	fD1	AGAGTTTGATCCTGGCTCAG	426	59	[17,24]
	16S rRNA	Rc16S.452n	AACGTCATTATCTTCCTTGC	426	59	[17,24]
	17 kDa	17 kDa-1	GCTCTTGCAACTTCTATGTT	434	58	[25]
	17 kDa	17 kDa-2	CATTGTTCGTCAGGTTGGCA	434	58	[25]
*Anaplasma/Ehrlichia* spp.	msp2	msp3F	CCAGCGTTTAGCAAGATAAGAG	334	56	[26]
	msp2	msp3R	GCCCAGTAACAACATCATAAGC	334	56	[26]
	16S rRNA (nested)	ge3a	CACATGCAAGTCGAACGGATTATTC	932	55	[27]
		ge10r	TTCCGTTAAGAAGGAT CTAATCTCC	932	55
		ge9f	AACGGATTATTCTTTATAGCTTGCT	546	55
		ge2	GGCAGTATTAAAAGCAGCTCCAGG	546	55
	16S rRNA	EHR 16SD	GGTACCYACAGAAGAAGTCC	345	55	[28]
	EHR	EHR 16SR	TAGCACTCATCGTTTACAGC	345	55	[28]
	16S rRNA	GEP-s	CTGGCGGCAAGCYTAACACATGCAAGTCGAACGGA	431	66	[29]
	GEP	GEP-as	CTTCTRTRGGTACCGTCATTATCTTCCCYAYTG	431	66	[29]
*Borrelia* spp.	flaB (nested)	Outer 1	AARGAATTGGCAGTTCAATC	497	52	[30]
		Outer 2	GCATTTTCWATTTTAGCAAGTGATG	497	52
		Inner 1	ACATATTCAGATGCAGACAGAGGTTCTA	389	55
		Inner 2	GAAGGTGCTGTAGCAGGTGCTGGCTGT	389	55
	5S-23S intergenic spacer (nested)	23SC1	TAAGCTGACTAATACTAATTACCC	380	52	[31]
		23SN1	ACCATAGACTCTTATTACTTTGAC	380	52
		5SCB	GAGAGTAGGTTATTGCCAGGG	226	55
		23SN2	ACCATAGACTCTTATTACTTTGACCA	226	55

bp: base pairs; Tm: melting temperature; Y = C/T; R = A/G; W = A/T.

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ISSN: 1756-3305

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