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Figure 2 | Parasites & Vectors

Figure 2

From: Identification and ultrastructural characterization of the Wolbachia symbiont in Litomosoides chagasfilhoi

Figure 2

Transmission electron microscopy revealing that the symbiotic bacterium is present in different tissues of Litomosoides chagasfilhoi. A – General view of the body wall showing the cuticle (C) and a bacterium (black arrowhead) present on the hypoderm (H) (Bar 2 μm). B – At high magnification, it is possible to observe a dividing bacterium on the hypoderm. It is worth noting that a classical septum is absent in the central constriction region (arrow) (Bar 5 μm). C and D – Transversal section showing the body wall composed of the cuticle (C), hypoderm (H), muscle layer (m) and proximal region of the ovary showing the ovary wall (Ow) and oocytes (Oc). At higher magnification, the endosymbiotic bacteria (arrowheads) are observed in the cytoplasmic oocytes and nucleus (N) (Bar = 50 μm in C and 20 mm in D). E – Longitudinal view of microfilariae (M) showing the nucleus (N) of sub-cuticular cells and endosymbiotic bacteria (arrowheads). Bar = 2 μm. F – At higher magnification, it is possible to note that in the microfilariae (M), the endosymbiont is inside a vacuole (arrowhead) close to the host cell nucleus (N) (Bar 0.5 μm.)

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