Figure 1

Isolation of Siba Def from the salivary gland of S. bannaense and MALDI–TOF MS. (A) The filtrate of the salivary gland homogenate of S. bannaense by 10 kDa cut-off was divided by an Inertsil C₄ RP-HPLC column (25 × 0.46 cm) equilibrated with 0.1% (v/v) trifluoroacetic acid/water. The elution was performed with the indicated gradient of acetonitrile at a flow rate of 1 ml/min. (B) The eluted peak of A2 containing antimicrobial activity was further purified by C₁₈ RP-HPLC column (25 × 0.46 cm) developed with a linear gradient of 0 to 70% acetonitrile in acidified water at a flow rate of 0.7 ml/min. The purified antimicrobial peptide is indicated by an arrow. (C) MALDI-TOF mass spectrometry analysis of the antimicrobial peptide.