Table 1 Primers used in the present study
Gene | Genbank accession | Primer sequences | Annealing temperature | Expected size (bp) | Ref. |
|---|---|---|---|---|---|
α-SMA | NM_007392.3 | F:5′-AAGAGCATCCGACACTGCTGAC-3′ | 60.0°C | 300 | Present study |
R:5′-AATAGCCACGCTCAGTCAGG-3′ | |||||
ColIa | NM_007742.3 | F: 5′-CAGGGTATTGCTGGACAACGTG-3′ | 60.0°C | 107 | Present study |
R: 5′-GGACCTTGTTTGCCAGGTTCA-3′ | |||||
Col-III | NM_009930.2 | F: 5′-TGGCACAGCAGTCCAACGTA-3′ | 60.0°C | 122 | Present study |
R: 5′-AAGGACAGATCCTGAGTCACAGACA-3′ | |||||
TGF-β1 | NM_011577 | F: 5′-GTGTGGAGCAACATGTGGAACTCTA-3′ | 60.0°C | 143 | 20 |
R: 5′-TTGGTTCAGCCACTGCCGTA-3′ | |||||
TGFβRI | NM_009370.2 | F: 5′-TGCAATCAGGACCACTGCAATAA-3′ | 60.0°C | 133 | 20 |
R: 5′-GTGCAATGCAGACGAAGCAGA-3′ | |||||
TGFβRII | NM_009371.2 | F: 5′-AAATTCCCAGCTTCTGGCTCAAC-3′ | 60.0°C | 100 | 20 |
R: 5′-TGTGCTGTGAGACGGGCTTC-3′ | |||||
Smad2 | NM_010754 | F: 5′-TGCATTCTGGTGTTCAATCG-3′ | 60.0°C | 198 | 20 |
R: 5′-CGAGTTTGATGGGTCTGTGA-3′ | |||||
Smad3 | NM_016769 | F: 5′-GTCAACAAGTGGTGGCGTGTG-3′ | 60.0°C | 150 | 20 |
R: 5′-GCAGCAAAGGCTTCTGGGATAA-3′ | |||||
Smad4 | NM_008540 | F: 5′-TGACGCCCTAACCATTTCCAG-3′ | 60.0°C | 136 | 20 |
R: 5′-CTGCTAAGAGCAAGGCAGCAAA-3′ | |||||
Smad7 | NM_001042660.1 | F: 5′-AGAGGCTGTGTTGCTGTGAATC-3′ | 60.0°C | 126 | 20 |
R: 5′-CCATTGGGTATCTGGAGTAAGGA-3′ | |||||
β-actin | NM_007393.3 | F: 5′-CGTGGGCCGCCCTAGGCACCA-3′ | 60.0°C | 243 | Present study |
R: 5′-TTGGCCTTAGGGTTCAGGGGGG-3′ |