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Table 3 Real time PCR validation of selected stage-biased expressed microRNAs in Echinococcus canadensis G7

From: microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach

  miRNA RNAseq p-adj 1 qPCR p-value 2
log 2 FC log 2 FC 3
CW-biased miR-4989-3p −2,76 <0,001 −2,62 <0,01
  miR-277-3p −2,70 <0,001 −2,98 <0,05
  miR-190-5p −2,66 <0,001 −1,35 <0,05
  miR-61-3p −1,50 <0,001 −1,34 <0,001
PS-biased mir-96-5p 1,87 <0,001 1,19 <0,01
  miR-7a-5p 3,20 <0,001 2,74 < 0,001
  miR-125-5p 4,35 <0,001 2,63 < 0,001
  1. 1P-adjusted values according to DESeq.
  2. 2P-values based on t-test.
  3. 3Correlation coefficient of log2 fold changes between RNAseq and RT-qPCR = 0.97.