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Table 3 Real time PCR validation of selected stage-biased expressed microRNAs in Echinococcus canadensis G7

From: microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach

 

miRNA

RNAseq

p-adj 1

qPCR

p-value 2

log 2 FC

log 2 FC 3

CW-biased

miR-4989-3p

−2,76

<0,001

−2,62

<0,01

 

miR-277-3p

−2,70

<0,001

−2,98

<0,05

 

miR-190-5p

−2,66

<0,001

−1,35

<0,05

 

miR-61-3p

−1,50

<0,001

−1,34

<0,001

PS-biased

mir-96-5p

1,87

<0,001

1,19

<0,01

 

miR-7a-5p

3,20

<0,001

2,74

< 0,001

 

miR-125-5p

4,35

<0,001

2,63

< 0,001

  1. 1P-adjusted values according to DESeq.
  2. 2P-values based on t-test.
  3. 3Correlation coefficient of log2 fold changes between RNAseq and RT-qPCR = 0.97.
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Parasites & Vectors

ISSN: 1756-3305

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