Bioinformatics analysis of Ts-clp. (A) Protein sequence analysis of Ts-CLP. Location of each region: signal peptide (s): 1–18; domain 1: 19–156; domain 2: 158–295; domain 3: 296–406; predicted cystatin motif (SSF54403): 161–262 and 305–381. The intron positions within Ts-clp are indicated by triangles. (B) Alignment of nematode cystatins and cystatin-like proteins. The predicted signal peptide of each sequence was excluded. Evolutionarily conserved sites are double underlined. The four cysteine residues in each domain of Ts-CLP are underlined. Potential auto-processing sites similar to GXNXFXD motif are shown in bold. Accession numbers for cDNA or proteins sequences are as follows: T. spiralis - Ts-CLP, ABY59464; Brugia malayi - Bm-CPI-1, U80972; Bm-CPI-2, AF015263; Onchocerca volvulus - Ov-CPI-1, AF177194; Ov-CPI-2, P22085; Litomosoides sigmodontis- Ls-CPI, AF229173; Acanthocheilonema viteae - Av-CPI, L43053; Nippostrongylus brasiliensis - Nb-CPI, AB050883; Haemonchus contortus - Hc-CPI AF035945; Caenorhabditis elegans -Ce-CPI-1, AF100663; Ce-CPI-2, AF068718.