Western blot analysis of Ts-CLP and its recombinant protein. (A) The antigenicity of rTs-CLP by Western blot analysis. M: protein standard; 1: sera of mice at 35 days p.i.; 2: sera of pigs at 26 days p.i.; 3: sera of rabbits immunised against rTs-CLP; 4: preimmune sera of pigs. (B) Western blot analysis of ES products using the sera of rTs-CLP immunised rabbits. M: protein standard; 1: purified rTs-CLP; 2–5: ES products of NBL, Ad5, ML, L6h.