Figure 4

Localisation of Ts-CLP in paraffin-embedded sections. Eight micrometre sections were treated with rabbit anti-rTs-CLP serum and Alexa Fluor 555 goat anti-rabbit IgG, then observed by confocal laser scanning microscopy. A: normal intestine; B: intestine epithelial tissue at 6 h p.i.; C: Ad3; D: Ad5; E: larvae in muscle at 9 days p.i.; F: larvae in muscle at 15 days p.i.; G: ML at 24 days p.i.; H: ML at 60 days p.i. The Ts-CLP signal was red. Bars = 20 μm. The white arrows indicate the worms of T. spiralis. The green arrows indicate the NBL of T. spiralis.