The complete mitochondrial genome of the gullet worm Gongylonema pulchrum: gene content, arrangement, composition and phylogenetic implications

Table 1 Primers used to amplify the mitochondrial genome of Gongylonema pulchrum in three overlapping long PCR fragments

Name of primer	Sequence (5′ to 3′)	Extension positions	Product lengths
cox3—cox2
GPcox3u	TATGATATATCTATTGATTATTG	4162	3954 bp
GPcox2d	GCATCCATCTTAATAAAACACTTAGG
cox2—cox1
GPcox2u	GAGGTCGATAATCGTTGTATTATCCCTGTGG	8013	7155 bp
GPcox1d	AAGAATGAATAACATCCGAAGAAGT	8013	7155 bp
cox1 —cox3
GPcox1u	TTTGGGGCTCCTGAGGTTTATA	770	3953 bp
GPcox3d	CAGAAATCTCTTCCATCACCTCGAT	770	3953 bp

ISSN: 1756-3305