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Table 3 Agreement in faecal egg counts (FECs) between initial reading and second quality control reading of Kato-Katz thick smears according to two different guidelines

From: Quality control in the diagnosis of Trichuris trichiura and Ascaris lumbricoides using the Kato-Katz technique: experience from three randomised controlled trials

 

WHO guideline a

Swiss TPH guideline a

 

Trichuris trichiura

Ascaris lumbricoides

Trichuris trichiura

Ascaris lumbricoides

Total number of Kato-Katz thick smears (positive/negative)

1,445 (1,189/256)

1,445 (297/1,148)

1,445 (1,189/256)

1,445 (297/1,148)

1. No. of Kato-Katz thick smears with difference in FECs (%)

345 (23.9%)

148 (10.2%)

97 (6.7%)

108 (7.5%)

2. No. of Kato-Katz thick smears with differences in presence/absence of helminth eggs (%)

51 (3.5%)

32 (2.2%)

51 (3.5%)

32 (2.2%)

3. No. of Kato-Katz thick smears with difference in FECs (%) among samples with egg counts

345 (29.0%)

148 (49.8%)

97 (8.2%)

108 (36.4%)

4. No. of Kato-Katz thick smears with differences in presence/absence of helminth eggs (%) among samples with egg counts

51 (4.3%)

32 (10.8%)

51 (4.3%)

32 (10.8%)

1. and/or 2. (%)

381 (26.4%)

164 (11.4%)

145 (10.0%)

130 (9.0%)

No. of Kato-Katz thick smears with discrepancies according to respective guidelineb (%)

345 (23.9%)

164 (11.4%)

145 (10.0%)

130 (9.0%)

3. and/or 4. and proportion as percentage (%) among Kato-Katz thick smears with positive egg counts

381 (32.0%)

164 (55.2%)

145 (12.2%)

130 (43.8%)

No. of Kato-Katz thick smears with discrepancies according to respective guidelineb (%) among Kato-Katz thick smears with positive egg counts

345 (29.0%)

148 (49.8%)

145 (12.2%)

130 (43.8%)

  1. aSee Table 1 for definition of the WHO and Swiss TPH guidelines.
  2. bThe WHO guideline state that differences which exceed 10% and more than four eggs require re-reading (see Table 1). However, in contrast to the Swiss TPH guideline, differences in presence/absence of helminth eggs are not explicitly stated. Therefore we assumed that differences in presence/absence of helminth eggs do not require re-reading as long as the difference does not exceed four eggs.