RNAi screen for mosquito SIT target genes. Ten gonad-specific dsRNAs were injected into pupae and 20 males were tested for fertility, fecundity, and mating competitiveness. A, Percentages of sterile males (blue bars) and fecundity of remaining, semi-fertile males (green bars), relative to negative control males, are indicated. B, Mating competitiveness of dsRNA-injected males was assessed in small (5 dsRNA males, 5 untreated males, 10 females) mating competitions by calculating percent viable progeny, relative to negative controls. Treatments not differing from the controls are marked (*; student t-test, P > 0.05). Error bars indicate the s.e.m. of three independent trials.