Figure 3

Seeding populations with dsRNA-treated mosquitoes reduces production of progeny. Larvae (first to fourth instars) were soaked daily for 1 h for 6 consecutive days in a mixture of dsx ^F and gas8-dsRNAs (0.2 mg/ml each). The adults arising from these treatments were mixed at different proportions with untreated males (100 males in total), and were mixed with 100 virgin females. The number of viable progeny from each mating cage was assessed 10 days later. Each point represents a single cage experiment (i.e. 6 replicates for each treatment), with the average number of progeny indicated (horizontal lines). Progressive increases in sterile male seeding density produced significantly greater reductions in the number of progeny produced (ANOVA, P < 0.01).