Silencing the buzz: a new approach to population suppression of mosquitoes by feeding larvae double-stranded RNAs

Parasites & Vectors

Table 3 Treatment of mosquito larvae with dsx ^F -dsRNA reduced production of females and all were sterile

dsRNA	dsRNA delivery	# Larvae treated ³	Females/males that developed	# Females that blood fed	# Females that produced progeny ⁴
gus	Daily soakings¹	420	207/200	162	139
dsx^F	Daily soakings¹	440	6/242	0	0
gus	Larval feeding²	445	238/187	194	172
dsx^F	Larval feeding²	460	7/241	1	0

¹Larvae (1^st-4^th instars) were soaked for 1 h/day for 6 days in 0.2 mg/ml dsRNA.
²Larvae were fed heat-killed E.coli continuously.
³Mixed sexes of larvae were treated.
⁴Females were provided 2 males for a period of one week, offered blood meals, and hatching of eggs monitored.

ISSN: 1756-3305