Figure 2

In situ hybridization (ISH) on Parvicapsula pseudobranchicola positive pseudobranchs from Atlantic salmon. A) Hematoxylin and Eosin stained tissue section covering both healthy and degenerate regions in the tissue and B) the result from in situ hybridization using the P. pseudobranchicola specific LNA probe on a neighbouring section of the same sample, showing specific staining in the same tissue region (20x magnification). C) and E) are enlarged sections from A) (40x magnification) with the corresponding results from ISH shown in D) and F), respectively. ISH with the LNA-modified polyT probe on a section from the same sample is shown in G), and H) shows ISH result in P. pseudobranchicola negative pseudobranch.