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Table 1 Molecular detection of rickettsiae, anaplasma and ehrlichia and blast analysis of the sequences derived from tick samples in this study

From: Molecular evidence of potential novel spotted fever group rickettsiae, Anaplasma and Ehrlichia species in Amblyomma ticks parasitizing wild snakes

Tick sample (Species, location)

Rickettsia

Anaplasma 16S rDNA

gltA

ompA

ompB

sca4

Candidatus Rickettsia sepangensis

     

S5(A. varanense, Sepang)

R. tamurae strain AT-1 (AF394896) (1033/1043, 99.0%)

R. tamurae strain AT-1 (DQ103259) (417/427, 97.7%)

Unable to be amplified

No significant similarity

A. phagocytophilum (AY551442, 99%, 253/256), A. platys (JX261979, 99% 253/256)

Candidatus Rickettsia johorensis

     

P1 (pooled A. helvolum, Johore), S4-2 (A. varanense, Sepang), S6-1 (A. helvolum, Sepang)

R. raoultii strain Khabarovsk (DQ365804) (1057/1060, 99.7%)

R. raoultii strain Khabarovsk (DQ365801) (418/429, 97.4%)

R. raoultii strain Khabarovsk (DQ365798) (762/775, 98.3%)

R. raoultii strain Khabarovsk (DQ365808) (795/816, 97.4%)

Not amplified

S7-2 (A. varanense, Sepang)

R. raoultii strain Khabarovsk (DQ365804) (1057/1060, 99.7%)

R. raoultii strain Khabarovsk (DQ365801) (418/429, 97.4%)

R. raoultii strain Khabarovsk (DQ365798) (762/775, 98.3%)

R. raoultii strain Khabarovsk (DQ365808) (795/816, 97.4%)

A. bovis (AB983438, 99%, 253/256)

S2, S4 (A. helvolum, Sepang), S6, S7 (A. varanense, Sepang)

Not amplified

A. phagocytophilum (AY551442, 99%, 253/256), A. platys (JX261979, 99%, 253/256)

S6-2 (A. varanense, Sepang)

Not amplified

A. bovis (AB983438, 99%, 253/256)

S3, S7-3 (A. varanense, Sepang)

Not amplified

Ehrlichia spp. (J410257, 99%, 249/256)

  1. The sequences obtained for rickettsiae from S5 and P1 ticks have been deposited in the GenBank database under the accession numbers: [gltA (GenBank: KJ769648, KJ769650), ompA (GenBank: KJ769649, KJ769651), ompB (GenBank: KJ769652), sca4 (GenBank: KM977711)].