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Table 1 Molecular detection of rickettsiae, anaplasma and ehrlichia and blast analysis of the sequences derived from tick samples in this study

From: Molecular evidence of potential novel spotted fever group rickettsiae, Anaplasma and Ehrlichia species in Amblyomma ticks parasitizing wild snakes

Tick sample (Species, location) Rickettsia Anaplasma 16S rDNA
gltA ompA ompB sca4
Candidatus Rickettsia sepangensis      
S5(A. varanense, Sepang) R. tamurae strain AT-1 (AF394896) (1033/1043, 99.0%) R. tamurae strain AT-1 (DQ103259) (417/427, 97.7%) Unable to be amplified No significant similarity A. phagocytophilum (AY551442, 99%, 253/256), A. platys (JX261979, 99% 253/256)
Candidatus Rickettsia johorensis      
P1 (pooled A. helvolum, Johore), S4-2 (A. varanense, Sepang), S6-1 (A. helvolum, Sepang) R. raoultii strain Khabarovsk (DQ365804) (1057/1060, 99.7%) R. raoultii strain Khabarovsk (DQ365801) (418/429, 97.4%) R. raoultii strain Khabarovsk (DQ365798) (762/775, 98.3%) R. raoultii strain Khabarovsk (DQ365808) (795/816, 97.4%) Not amplified
S7-2 (A. varanense, Sepang) R. raoultii strain Khabarovsk (DQ365804) (1057/1060, 99.7%) R. raoultii strain Khabarovsk (DQ365801) (418/429, 97.4%) R. raoultii strain Khabarovsk (DQ365798) (762/775, 98.3%) R. raoultii strain Khabarovsk (DQ365808) (795/816, 97.4%) A. bovis (AB983438, 99%, 253/256)
S2, S4 (A. helvolum, Sepang), S6, S7 (A. varanense, Sepang) Not amplified A. phagocytophilum (AY551442, 99%, 253/256), A. platys (JX261979, 99%, 253/256)
S6-2 (A. varanense, Sepang) Not amplified A. bovis (AB983438, 99%, 253/256)
S3, S7-3 (A. varanense, Sepang) Not amplified Ehrlichia spp. (J410257, 99%, 249/256)
  1. The sequences obtained for rickettsiae from S5 and P1 ticks have been deposited in the GenBank database under the accession numbers: [gltA (GenBank: KJ769648, KJ769650), ompA (GenBank: KJ769649, KJ769651), ompB (GenBank: KJ769652), sca4 (GenBank: KM977711)].