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Table 1 Sequences of primers used to amplify fragments from Hypoderaeum conoideum

From: Mitochondrial genome of Hypoderaeum conoideum – comparison with selected trematodes

Primer codes

Sequences(5′ to 3′)

Target gene

XCCOX3F2

AGYACDGTDGGDTTRCATTT

cox31

XCCOX3R1

CANAYATAATCMACARAATGNCA

cox31

XcND4F

GADTCBCCDTATTCDGARCG

nad41

XcND4R

GCHARCCADCGCTTVCCNTC

nad41

TXCCOX1F

GGHTGAACHRTWTAYCCHCC

cox11

TXCCOX1R

TGRTGRGCYCAWACDAYAMAHCC

cox11

Insect12SF

AAWAAYGAGAGYGACGGGCG

rrnS1

Insect12SR

TARACTAGGATTAGATACCC

rrnS1

XcND5F

ATGCGNGCYCCNACNCCNGTDAG

nad51

XcND5R1

TGCTTVSWAAAAAANACHCC

nad51

XCF2

TATTAGGAGGTTTGGTGG

cox3-nad42

XCR3

ATCATAACTACCACATACCCC

cox3-nad42

XCF4

TAGGTATTGCTTGTTAGCTG

nad4-cox12

XCR2

TTTAATCGAACCAAGGACAC

nad4-cox12

XCF3

CATTAGTCACATTTGTATGAC

cox1- rrnS2

XCR10

GGACTATCTTTTATGATACACG

cox1- rrnS2

XCF1

GTTATTGGGTTTAGGACTCGG

rrnS - nad52

XCR8

ACTAACACCGTATTCAACTC

rrnS - nad52

XCF9

TTTCTCTTTGTGGTTTGCCG

nad5-cox32

XCR1

TATTAGGTTGTGGTACCCC

nad5-cox32

  1. Primer pairs (top to bottom) used to amplify fragments; 1short regions amplified by PCR from cox1 (494 bp), cox3 (140 bp), nad4 (440 bp), nad5 (529 bp) and rrnS (383 bp). 2large fragments that were amplified by long-range PCR from cox3-nad4 (2048 bp), nad4-cox1 (4664 bp), cox1-rrnS (2352 bp), rrnS-nad5 (2272 bp) and nad5-cox3 (1752 bp).
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Parasites & Vectors

ISSN: 1756-3305

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