Figure 2

IgG profile of serum antibodies to Leishmania ( L.) infantum chagasi of CD43-deficient mice. Sera of CD43^+/+ and CD43^−/− mice infected with 5 × 10⁷ amastigote forms of Leishmania (L.) infantum chagasi were collected at 30 DPI and the absorbance values of IgG antibody isotypes (IgG1, IgG2a and IgG2b) were determined by ELISA using Leishmania (L.) infantum chagasi promastigote lysates. Bars show levels of IgG1, IgG2a and IgG2b antibodies as the individual absorbancy values of 1/100 diluted sera. Asterisks indicate significant differences between groups ***(p < 0.001). Data represent the individual results for each group of mice of two independent experiments.