Expression and purification of the recombinant protein of RHcyst-
a novel type 1 cystatin isolated from the tick
in a GST-
fused soluble form,
. A total of ~10 μg recombinant protein or bacterial lysate was electrophoresed in a 12% SDS-polyacrylamide gel, and then stained with Coomassie blue. Lane 1: The supernatant of uninduced cell lysate; lane 2: The supernatant of induced cell lysate; and lanes 3 and 4: purified RHcyst-1 recombinant protein.