Inhibition of protease activities by the recombinant protein of RHcyst-
a novel type 1 cystatin isolated from the tick
. Cathepsin L, B, C, H, and S and papain were incubated with each of the substrates in the presence of different concentrations of rRHcyst-1. Incubation of cathepsins without rRHcyst-1 resulted in 100% enzyme activity.