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Table 1 Protease inhibition assays

From: Functional characterization of a cystatin from the tick Rhipicephalus haemaphysaloides

Enzyme Enzyme concentration RHcyst-1 IC 50 and 95% confidence intervals
Papain 150 nM 89 nM (86 to 92.1 nM)
Cathepsin B 150 nM 594.5 nM (305.5 to 1157 nM)
Cathepsin L 150 nM 34 nM (9.2 to 125.5 nM)
Cathepsin C 150 nM 281.8 nM (244.5 to 324.7 nM)
Cathepsin H 150 nM 23.7 nM (22.2 to 25.4 nM)
Cathepsin S 150 nM 0.3 nM (~ to 1.4 nM)
  1. The concentration of a novel type 1 cystatin, RHcyst-1, isolated from the tick Rhipicephalus haemaphysaloides, at which 50% of the proteolytic enzymes’ activity is inhibited (IC50).