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Figure 2 | Parasites & Vectors

Figure 2

From: Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct

Figure 2

Expression pattern of Cs ACAT. (A) Immunolocalization of CsACAT in C. sinensis adult. Panel a and b, sections treated with anti-rCsACAT serum and specific fluorescences distributed in the vitellarium and sub-tegumental muscular layer of the adult worm; Panel c and d, sections treated with naive serum. No fluorescence was detected. M, sub-tegumental muscular layer; V, vitellarium. Magnification for the adult worm was × 100. (B) mRNA level of CsACAT at different developmental stages of C. sinensis by quantitative real-time PCR. The specific mRNA fragment of CsACAT was observed among the stages. The quantities were normalized with Cs β-actin and analyzed by means of the 2−ΔΔCt ratio.

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