Figure 5

Worm burden in rabbit models and expression level of Cs ACAT in the worms. (A) Worm burden in hypercholesteremia or basal diet fed rabbits 6 weeks after C. sinensis infection. All data are presented as mean ± SD, *p < 0.05, **p < 0.01. (B) The mRNA level of CsACAT in adult worms extracted from hypercholesteremia or basal diet fed rabbits, normalized with Cs β-actin. (C) The protein levels of CsACAT in adult worms of the two groups. Lane 1–3, total proteins of worms recovered from basal diet treated rabbits probed with rat anti-rCsACAT serum. Lane 4–6, total proteins of worms recovered from hypercholesteroemic rabbits blotted with rat anti-rCsACAT serum. (D) Optical density analysis of the protein levels in Figure 5C. The optical density was calculated by Tanon Gis software and analyzed by Student’s t test, *p < 0.05.