Genotype diversity of Trypanosoma cruzi in small rodents and Triatoma sanguisuga from a rural area in New Orleans, Louisiana

Table 1 Primers used for the detection and genotyping of Trypanosoma cruzi. Primer

	Sequence	Target	Reference
S35	5-’AAA TAA TGT ACG GGK GAG ATG CAT GA	Minicircle variable region of kDNA	[21]
S36	5’-GGG TTC GAT TGG GGT TGG TGT	Minicircle variable region of kDNA	[21]
TcZ1	5’-CGA GCT CTT GCC CAC ACG GGT GCT	Nuclear satellite DNA	[22]
TcZ2	5’CCT CCA AGC AGC GGA TAG TTC AGG	Nuclear satellite DNA	[22]
Tc1	5’-ACA CTT TCT GTG GCG CTG ATC G	Miniexon intergenic region	[23]
Tc2	5’-TTG CTC GCA CAC TCG GCT GCA T
Tc3	5’-CCG CGW ACA ACC CCT MAT AAA AAT G
Tr	5’-CCT ATT GTG ATC CCC ATC TTC G
Me	5’-TAC CAA TAT AGT ACA GAA ACT G
D71	5’-AAG GTG CGT CGA CAG TGT GG	D7 divergent domain of the 24Sα rRNA gene	[24]
D72	5’-TTT TCA GAA TGG CCG AAC AGT	D7 divergent domain of the 24Sα rRNA gene	[24]
V1	5’-CAA GCG GCT GGG TGG TTA TTC CA	Size-variable domain of the 18S rRNA gene	[25]
V2	5’-TTG AGG GAA GGC ATG ACA CAT GT		[25]

ISSN: 1756-3305