Long-term experiment showing
numbers in the gut of
. Parasite numbers were analysed in R. prolixus
(A) 4th and (B) 5th instar nymphs: anterior midgut and posterior midgut at 2 and 7 days after feeding. Fourth instar nymphs were fed on inactivated blood containing 1 × 106 epimastigotes/mL. After moulting, 5th instar nymphs were fed on blood without parasites. Bars represent mean ± SEM of three independent experiments each with five insects (n = 15). Means were compared using one-way ANOVA and Kruskal-Wallis test; ***p < 0.001 and *p < 0.05.