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Figure 2 | Parasites & Vectors

Figure 2

From: A reduced VWA domain-containing proteasomal ubiquitin receptor of Giardia lamblia localizes to the flagellar pore regions in microtubule-dependent manner

Figure 2

In vitro ubiquitin binding assay of UIM of GlRpn10 subunit. UIM of GlRpn10 and ScVps27 were tagged with 6XHis tag. The isolated proteins were immobilized on Ni-NTA agarose beads and allowed to bind GST-tagged ubiquitin. Following elution with imidazole, the eluate was analyzed by Western blotting with anti-GST antibody. GlUIM* represents S → E mutant protein of GlRpn10 UIM. One-fifth of the eluate volume used for the anit-GST blot, was run on a separate gel and stained with coomassie blue.

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