Figure 4From: Rhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response Activity inhibition assay of cathepsins B, C, and L by rJpIocys2a, rBrBmcys2b, and rBrBmcys2c. Cathepsins B, C, and L were incubated with Z-Arg-Arg-pNA (0.125Â mM), Gly-Phe-pNA (1.8Â mM), or Z-Phe-Arg-MCA (0.02Â mM), respectively, in the presence of different concentrations of rJpIocys2a, rBrBmcys2b, and rBrBmcys2c. The abscissa shows inhibitors concentration (nM, log10); the ordinate shows percentage of remaining enzymatic activity. Incubation of cathepsins B, C, and L without rJpIocys2a, rBrBmcys2b, and rBrBmcys2c represents 100% of enzyme activity.Back to article page