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Table 1 Cystatins dissociation constants (KIs) for different proteases

From: Rhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response

Enzyme

Family

Enzyme concentration

Ki (nM)

rBrBmcys2b

rBrBmcys2c

rJpIocys2a

Cathepsin B 1

Cysteine protease

0.500 μM

0.82 ± 0.35

n.i.

154.70 ± 106.71

Cathepsin C 1

Cysteine protease

0.160 μM

26.65 ± 6.75

0.45 ± 0.12

>1 μM

Cathepsin L 2

Cysteine protease

0.043 μM

2.48 ± 1.00

28.45 ± 2.34

4.39 ± 1.76

Cathepsin G 2

Serine protease

0.100 μM

n.i

n.i.

n.i.

  1. n.i., not inhibited in the presence of 0.5 μM recombinant cystatin.
  2. 1Bovine cathepsin.
  3. 2Human cathepsin.