Table 1 Targeted genes and list of primers used in this study
Pathogen | Gene | Primer | Size (bp) | Location in locus/gene | Fragment length (bp) | References |
|---|---|---|---|---|---|---|
Anaplasma spp./Ehrlichia spp. | 16S | E.c-16S fwd | TCGCTATTAGATGAGCCTACGT | 285-408 | 123 | |
E.c-16S rev | GAGTCTGGACCGTATCTCAGT | |||||
Anaplasma spp./Ehrlichia spp. | 16S | EHR16SD | TAGCACTCATCGTTTACAGC | 525-870 | 345 | [30] |
EHR16SR | GGTACCYACAGAAGAAGTCC | |||||
Leishmania spp. | ITS | ITS-219F | AGCTGGATCATTTTCCGATG | 219-484 | 265 | [31] |
ITS-219R | ATCGCGACACGTTATGTGAG |