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Table 2 Primers sets for PCR amplification of CVBD agents

From: Bacterial and protozoal agents of canine vector-borne diseases in the blood of domestic and stray dogs from southern Portugal

Pathogen Primers Product size (bp) Reference
Anaplasma spp./Ehrlichia spp. EHR16SD: 5'-GGT ACC YAC AGA AGA AGTCC-3' 345 [27]
EHR16SR: 5'-TAG CAC TCA TCG TTT ACAGC-3'
Bartonella spp. 325 s: 5'-CTTCAGATGATGATCCCAAGCCTTCTGGCG-3' 500-800 [28]
1100as: 5'-GAACCGACGACCCCCTGCTTGCAAAGCA-3'
Borrelia burgdorferi s.l. Outer primers: 774 [29]
132f: 5'-TGGTATGGGAGTTTCTGG-3'  
905r: 5'-TCTGTCATTGTAGCATCTTT-3'  
Inner primers: 604 [29]
220f: 5'-CAGACAACAGAGGGAAAT-3'  
823r: 5'-TCAAGTCTATTTTGGAAAGCACC-3'  
Babesia spp. PIRO-A: 5'-AAT ACC CAA TCC TGA CACAGG G-3' 400 [27]
PIRO-B: 5'-TTA AAT ACG AAT GCC CCCAAC-3'
Hepatozoon spp. HEP-F: 5'-ATA CAT GAG CAA AAT CTC AAC-3' 626-666 [30]
HEP-R: 5'-CTT ATT ATT CCA TGC TGC AG-3'
Leishmania infantum MC1: 5’-GTTAGCCGATGGTGGTCTTG-3’ 447 [31]
MC2: 5’-CACCCATTTTTCCGATTTTG-3’