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Table 3 Infectiousness of BALB/c mice for Phlebotomus orientalis : second series of xenodiagnoses

From: Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: a new laboratory model

Mouse No.

Site of feeding of sand flies

Weeks post-infection

9

10

11

12

15

No fed females

No (%) positive

No fed females

No (%) positive

No fed females

No (%) positive

No fed females

No (%) positive

No fed females

No (%) positive

#1

Inoculated ear

13

1 (7.7)a

9

0

21

3 (14.3)b

15

1 (6.7)c

6

0

Contralateral ear

3

0

13

0

6

0

18

0

5

0

Front paws

8

0

7

0

4

0

9

0

9

0

Hind paws and tail

28

0

7

0

12

0

9

0

10

0

#2

Inoculated ear

20

4 (20.0)d

6

0

12

1 (8.3)e

14

0

8

0

Contralateral ear

4

0

16

0

10

0

13

0

5

0

Front paws

7

0

6

0

9

0

14

0

15

0

Hind paws and tail

4

0

5

0

3

0

9

0

10

0

#5

Inoculated ear

23

5 (25.0)f

28

3 (10.7)g

27

10 (37.0)h

19

5 (26.3)i

41

3 (7.3)j

Contralateral ear

11

0

24

0

40

0

12

0

10

0

Front paws

3

0

17

0

17

0

13

0

11

0

Hind paws and tail

19

0

15

0

29

0

5

0

10

0

#7

Inoculated ear

20

2 (13.3)k

18

2 (11.1)l

16

0 (0)

18

6 (33.3)m

11

1 (9.0)n

Contralateral ear

14

0

22

0

20

0

8

pos.

14

0

Front paws

3

0

7

0

9

0

6

0

5

0

Hind paws and tail

0

0

26

0

6

0

8

0

10

0

#9

Inoculated ear

15

8 (34.7)o

35

7 (20.0)p

18

4 (22.2)q

20

9 (45.0)r

13

1 (7.7)s

Contralateral ear

20

0

12

0

17

0

27

pos.

8

0

Front paws

9

0

6

0

3

0

9

0

8

0

Hind paws and tail

8

0

23

0

9

0

32

0

5

0

∑

Inoculated ear

91

20 (22.0)

96

12 (12.5)

94

18 (19.1)

86

21 (24.4)

79

5 (6.3)

Contralateral ear

52

0

87

0

93

0

78

2/5 pools

42

0

Front paws

30

0

43

0

42

0

51

0

48

0

Hind paws and tail

59

0

76

0

59

0

63

0

45

0

  1. Engorged sand flies were maintained on 50% aqueous sucrose solution till day 2 post-blood meal at 26°C and then tested by qPCR for presence and quantity of Leishmania donovani DNA. Sand flies fed on the inoculated ear were tested individually; sand flies fed on other parts of mice body were pooled for PCR analysis.
  2. p.i., post infection, pos. = positive pool, a-s, numbers of Leishmania detected by qPCR: a, 320; b, all <10; c, <10; d, 1652, 419, 643, 297; e, 430; o, 708, 21, 245, 27, 476, 4035, 24, 68; p, 1850, 211, 39, 3216, 74, 48, 162; q, 18, 143, 2161, 614; r, 20, 481, 5031, 50, 21, 171, 572, 228, 17; s, 279; f, 153, 113, 168, 404, 665; g, 69, 1286, 517; h, 1574, 37, 30, 11085, 1021, 15, 2844, 137, 64, 1815; i, 425, 100, 444, 1917, 2186; j, 273, 860, 50; k; 143, 180; l, 60, 1333; m, 146, 382, 40049, 66, 151, 1123; n, 256.
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