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Table 3 Infectiousness of BALB/c mice for Phlebotomus orientalis : second series of xenodiagnoses

From: Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: a new laboratory model

Mouse No. Site of feeding of sand flies Weeks post-infection
9 10 11 12 15
No fed females No (%) positive No fed females No (%) positive No fed females No (%) positive No fed females No (%) positive No fed females No (%) positive
#1 Inoculated ear 13 1 (7.7)a 9 0 21 3 (14.3)b 15 1 (6.7)c 6 0
Contralateral ear 3 0 13 0 6 0 18 0 5 0
Front paws 8 0 7 0 4 0 9 0 9 0
Hind paws and tail 28 0 7 0 12 0 9 0 10 0
#2 Inoculated ear 20 4 (20.0)d 6 0 12 1 (8.3)e 14 0 8 0
Contralateral ear 4 0 16 0 10 0 13 0 5 0
Front paws 7 0 6 0 9 0 14 0 15 0
Hind paws and tail 4 0 5 0 3 0 9 0 10 0
#5 Inoculated ear 23 5 (25.0)f 28 3 (10.7)g 27 10 (37.0)h 19 5 (26.3)i 41 3 (7.3)j
Contralateral ear 11 0 24 0 40 0 12 0 10 0
Front paws 3 0 17 0 17 0 13 0 11 0
Hind paws and tail 19 0 15 0 29 0 5 0 10 0
#7 Inoculated ear 20 2 (13.3)k 18 2 (11.1)l 16 0 (0) 18 6 (33.3)m 11 1 (9.0)n
Contralateral ear 14 0 22 0 20 0 8 pos. 14 0
Front paws 3 0 7 0 9 0 6 0 5 0
Hind paws and tail 0 0 26 0 6 0 8 0 10 0
#9 Inoculated ear 15 8 (34.7)o 35 7 (20.0)p 18 4 (22.2)q 20 9 (45.0)r 13 1 (7.7)s
Contralateral ear 20 0 12 0 17 0 27 pos. 8 0
Front paws 9 0 6 0 3 0 9 0 8 0
Hind paws and tail 8 0 23 0 9 0 32 0 5 0
Inoculated ear 91 20 (22.0) 96 12 (12.5) 94 18 (19.1) 86 21 (24.4) 79 5 (6.3)
Contralateral ear 52 0 87 0 93 0 78 2/5 pools 42 0
Front paws 30 0 43 0 42 0 51 0 48 0
Hind paws and tail 59 0 76 0 59 0 63 0 45 0
  1. Engorged sand flies were maintained on 50% aqueous sucrose solution till day 2 post-blood meal at 26°C and then tested by qPCR for presence and quantity of Leishmania donovani DNA. Sand flies fed on the inoculated ear were tested individually; sand flies fed on other parts of mice body were pooled for PCR analysis.
  2. p.i., post infection, pos. = positive pool, a-s, numbers of Leishmania detected by qPCR: a, 320; b, all <10; c, <10; d, 1652, 419, 643, 297; e, 430; o, 708, 21, 245, 27, 476, 4035, 24, 68; p, 1850, 211, 39, 3216, 74, 48, 162; q, 18, 143, 2161, 614; r, 20, 481, 5031, 50, 21, 171, 572, 228, 17; s, 279; f, 153, 113, 168, 404, 665; g, 69, 1286, 517; h, 1574, 37, 30, 11085, 1021, 15, 2844, 137, 64, 1815; i, 425, 100, 444, 1917, 2186; j, 273, 860, 50; k; 143, 180; l, 60, 1333; m, 146, 382, 40049, 66, 151, 1123; n, 256.