|
Mouse No.
|
Site of feeding of sand flies
|
Weeks post-infection
|
|---|
|
9
|
10
|
11
|
12
|
15
|
|---|
|
No fed females
|
No (%) positive
|
No fed females
|
No (%) positive
|
No fed females
|
No (%) positive
|
No fed females
|
No (%) positive
|
No fed females
|
No (%) positive
|
|---|
|
#1
|
Inoculated ear
|
13
|
1 (7.7)a
|
9
|
0
|
21
|
3 (14.3)b
|
15
|
1 (6.7)c
|
6
|
0
|
|
Contralateral ear
|
3
|
0
|
13
|
0
|
6
|
0
|
18
|
0
|
5
|
0
|
|
Front paws
|
8
|
0
|
7
|
0
|
4
|
0
|
9
|
0
|
9
|
0
|
|
Hind paws and tail
|
28
|
0
|
7
|
0
|
12
|
0
|
9
|
0
|
10
|
0
|
|
#2
|
Inoculated ear
|
20
|
4 (20.0)d
|
6
|
0
|
12
|
1 (8.3)e
|
14
|
0
|
8
|
0
|
|
Contralateral ear
|
4
|
0
|
16
|
0
|
10
|
0
|
13
|
0
|
5
|
0
|
|
Front paws
|
7
|
0
|
6
|
0
|
9
|
0
|
14
|
0
|
15
|
0
|
|
Hind paws and tail
|
4
|
0
|
5
|
0
|
3
|
0
|
9
|
0
|
10
|
0
|
|
#5
|
Inoculated ear
|
23
|
5 (25.0)f
|
28
|
3 (10.7)g
|
27
|
10 (37.0)h
|
19
|
5 (26.3)i
|
41
|
3 (7.3)j
|
|
Contralateral ear
|
11
|
0
|
24
|
0
|
40
|
0
|
12
|
0
|
10
|
0
|
|
Front paws
|
3
|
0
|
17
|
0
|
17
|
0
|
13
|
0
|
11
|
0
|
|
Hind paws and tail
|
19
|
0
|
15
|
0
|
29
|
0
|
5
|
0
|
10
|
0
|
|
#7
|
Inoculated ear
|
20
|
2 (13.3)k
|
18
|
2 (11.1)l
|
16
|
0 (0)
|
18
|
6 (33.3)m
|
11
|
1 (9.0)n
|
|
Contralateral ear
|
14
|
0
|
22
|
0
|
20
|
0
|
8
|
pos.
|
14
|
0
|
|
Front paws
|
3
|
0
|
7
|
0
|
9
|
0
|
6
|
0
|
5
|
0
|
|
Hind paws and tail
|
0
|
0
|
26
|
0
|
6
|
0
|
8
|
0
|
10
|
0
|
|
#9
|
Inoculated ear
|
15
|
8 (34.7)o
|
35
|
7 (20.0)p
|
18
|
4 (22.2)q
|
20
|
9 (45.0)r
|
13
|
1 (7.7)s
|
|
Contralateral ear
|
20
|
0
|
12
|
0
|
17
|
0
|
27
|
pos.
|
8
|
0
|
|
Front paws
|
9
|
0
|
6
|
0
|
3
|
0
|
9
|
0
|
8
|
0
|
|
Hind paws and tail
|
8
|
0
|
23
|
0
|
9
|
0
|
32
|
0
|
5
|
0
|
|
∑
|
Inoculated ear
|
91
|
20 (22.0)
|
96
|
12 (12.5)
|
94
|
18 (19.1)
|
86
|
21 (24.4)
|
79
|
5 (6.3)
|
|
Contralateral ear
|
52
|
0
|
87
|
0
|
93
|
0
|
78
|
2/5 pools
|
42
|
0
|
|
Front paws
|
30
|
0
|
43
|
0
|
42
|
0
|
51
|
0
|
48
|
0
|
|
Hind paws and tail
|
59
|
0
|
76
|
0
|
59
|
0
|
63
|
0
|
45
|
0
|
- Engorged sand flies were maintained on 50% aqueous sucrose solution till day 2 post-blood meal at 26°C and then tested by qPCR for presence and quantity of Leishmania donovani DNA. Sand flies fed on the inoculated ear were tested individually; sand flies fed on other parts of mice body were pooled for PCR analysis.
- p.i., post infection, pos. = positive pool, a-s, numbers of Leishmania detected by qPCR: a, 320; b, all <10; c, <10; d, 1652, 419, 643, 297; e, 430; o, 708, 21, 245, 27, 476, 4035, 24, 68; p, 1850, 211, 39, 3216, 74, 48, 162; q, 18, 143, 2161, 614; r, 20, 481, 5031, 50, 21, 171, 572, 228, 17; s, 279; f, 153, 113, 168, 404, 665; g, 69, 1286, 517; h, 1574, 37, 30, 11085, 1021, 15, 2844, 137, 64, 1815; i, 425, 100, 444, 1917, 2186; j, 273, 860, 50; k; 143, 180; l, 60, 1333; m, 146, 382, 40049, 66, 151, 1123; n, 256.
